مجله دانش و تندرستی در علوم پایه پزشکی
سال چهاردهم شماره 2 (تابستان 1398)

Today, the production of biological nanoparticles has expanded significantly in different industries. Although their side effects have not yet been fully evaluated on brain tissue. In addition, by applying a stressor stimulus less than the threshold for injury to the tissue (pre-preparation), such as physical activity, it can increase the resistance of the tissue to more powerful stimuli. Therefore, the purpose of this study was to investigate the effect of high dose doses of biological nanoparticles with two different types of training prediction on structural changes in brain tissue of male Wistar rats.

A total of 30 male Wistar rats were divided into 6 groups including healthy control, silver biological nanoparticles, aerobic exercise protocols, training protocols 2 (anaerobes), biological biological nanoparticles + exercise protocols 1, biological nanoparticles Silver + Practice Protocol 2 was done. Intraperitoneal injection of silver nanoparticles after 10 weeks of aerobic and anaerobic treatments was performed on 10% of body weight of each rat in 5 times. After 48 hours after the last injection, rats were indifferent and sample was taken. The specimens were then photographed and stained with Hematoxylin-Eosin stained with optical microscopy.

The results of this study showed that aerobic training and anaerobic exercise training had a significant effect on the distance traveled in the progressive endurance test on rodent treadmill (P=0.000) and body weight (P=0.000). Intraperitoneal injection of high-dose silver nanoparticles also caused inflammation and mild inflammation of brain tissue in untreated rats, and the white matter and gray matter of nerve cells did not change. On the other hand, it was reduced trained groups and in the aerobic training group it was more significant than anaerobic.

Pre-conditioning of aerobic training can be effective in reducing the degree of brain tissue damage caused by the injection of silver biologic nanoparticles.

The parasitic diseases are contained of wide ranges of zoonosis diseases which not only causing dimension harms to animal husbandry industry and corps elimination and polluted organs but also are cause of diseases in humans, between them Fasciolosis, Hydatidosis, polluting to Dicrocoelium dendriticum and Cysticercosis could be pointed. study of slaughtered cattles polluted by Hydatid cyst, Cysticercosis, Fasciola Spp, and Dicrocoelium in slaughterhouse in Sarpul-e-zahab (Kermanshah).

This study is cross-sectional descriptive and was carried out by macroscopic observation and 1170 cattle have been studied. The outcome data was analyzed by statistical software SPSS and chi square.

That was reported there was 35.64%(417 cases) polluted cattle between 1170 cattle. Pollution rates to Fasciola, Dicrocoelium, Hydatid cyst and Cysticercus were 5.47% (64 cases), 0.85% (10 cases), 8.11% (95 cases) and 0.86% (8 cases) respectively, and lung pollution rates to Hydatid cyst and Cysticercus were reported 20.17% (236 cases) and 0.34% (4 cases) respectively. There were 973 bulls and 197 cows between 1170 cattle, 35.66% (347 cases) of bulls and 35.53% (70 cases) were polluted. According to the age pollution rates were analyzed in under one age group (935 cases) and more or equal than one (235 cases) which pollution percent of them were 35.61% (333 cases) and 35.74% (84 cases) respectively.
There is no significant statistical difference between age and sex and positive pollution cases.

findings of this study shows high rate pollution of area which harms of diseases and corpse elimination and polluted organs in slaughterhouses may cause pollution of humans, so using the adaptable approaches for control and prevention of polluting the animal husbandry is very important..

Methamphetamine is one of a number of artificial stimulants that can cause irreversible damage to the central nervous system. Recent studies have shown that methamphetamine result in neuronal damage through several ways like effecting on brain neurotransmitters such as dopamine, increased free radicals and oxidative stress, accelerated cell death, and apoptosis. In addition, there are many studies and evidence of anti-inflammatory, anti-apoptotic and antioxidant effects of hydrogen sulfide on various types of neurological diseases. Therefore, this study was designed to help prove the function of sulfide hydrogen neurotransmitter protection against neurotoxic activity of methamphetamine.

Methamphetamine neurotoxicity was induced by 40 mg/kg of METH in four intraperitoneally (IP) injections (e.g., 4×10 mg/kg q. 2-h, IP.). NaHS was administered at 30- min, 24-h, and 48 h after the final injection of METH. Spatial memory test was evaluated by Morris water maze then the brains were removed for Nissl staining to assess necrosis neuronal death within the hippocampal CA1 area

Behavioral tests shows that H2S treatment could significantly improve spatial memory deficits and learning (P<0.05) versus the METH group. Moreover, H2S could significantly reduce necrosis cell death (P<0.01) in CA1 area of hippocampus

According to the findings, H2S exerts significant neuroprotective effects on METH neurotoxicity due to its antioxidant and anti-inflammatory activity.

Lead is a heavy metal that causes toxicity in various tissues, including kidneys. The aim of this study was to evaluate the protective effects of ascorbic acid on lead-induced renal toxicity.

24 rats were divided into three groups: 1- Control group that consumed normal water, 2- Lead acetate group (mg/liter 500) in drinking water and 3- Lead acetate + Ascorbic acid group (1 gr/liter). After 8 weeks, the rats were completely anesthetized and blood and kidney tissues were collected.

Lead acetate caused increase in BUN and creatinine compared to control group. In addition, in Lead acetate group malondialdehyde (MDA) was elevated and glutathione (GSH) was decreased in renal tissues. In Lead acetate + Ascorbic acid group, MDA was decreased and GSH was increased in contrast to Lead acetate group.

According to this data, oral ascorbic acid consumption reduces the renal functional injury and oxidative stress following lead toxicity.

Streptomyces are microorganisms that are present in a variety of natural resources, especially in the soil, and can produce a wide variety of antimicrobial compounds. The prevalence of microbial resistance among pathogen microorganisms is increasing. The purpose of this study was to isolate and identify Streptomyces producing antimicrobial compounds with molecular methods and optimize its production conditions.

Streptomyces isolated from the soils of different regions of eastern Gilan province. Then, morphology, physiology and biochemical identification of the isolated samples were investigated. Finally, the identification of the isolates was carried out using sequencing of 16 SrRNA and phylogenetic analysis. Antimicrobial activity against tested pathogenic microorganisms and optimization of antimicrobial compounds produced in nutrient media and different conditions such as carbon and nitrogen sources, pH, incubation time and temperature were performed.

Based on the results of phylogenetic studies and sequencing of 16SrRNA, 97.34% confidence was detected in Streptomyces tendae strain 944. The bacterium had antimicrobial activity against pathogenic microorganisms including Micrococcus luteus PTCC 1408, Bacillus cereus PTCC 1154, Staphylococcus aureus PTCC 1189, Pseudomonas aeruginosa PTCC 1310, Salmonella typhi PTCC 1609 and Proteus mirabilis PTCC 1776. After optimization, the best culture medium, pH, optimum temperature, carbon and nitrogen sources and incubation time were ISP2, 7, 28 0 C, glucose, yeast extract and 7 days respectively.

The first report of screening of Streptomyces producing bioactive compounds in the eastern regions of Guilan province, show that the soils of the eastern regions of Guilan province are a very rich source of streptomyces producing antimicrobial compounds that can it is very important to produce natural-antimicrobial compounds.

The most common endocrine disorder in women of infertility is polycystic ovary syndrome. Increased oxidative stress is one of the pathogenesis of this disease. Therefore, combating oxidative stress is helpful in reducing the complications of this disease. In the present study, the effects of vitamin E, C and selenium supplementation on the induction of polycystic ovary induced rat model were investigated.

Thirty female Wistar rats were divided randomly into 6 groups. Induction of polycystic ovary syndrome was performed with letrozole gavage. The groups were then treated with vitamin C, vitamin E, selenium, and a mixture of vitamins C, E, and Se for one month. The control group received normal saline during the study period. The mice were sacrificed after one month and their blood was taken for oxidative stress tests including total antioxidant capacity and free oxygen species and malondialdehyde. Their ovaries were also removed for pathological examination and transferred to formalin.

The results showed that there was an increase in oxidative stress in the polycystic ovary induction group (P<0.05). The results also showed that the group with the simultaneous supplementation of vitamin C, E, Se and Se had the highest decrease in oxidative stress indices (P<0.003). Also vitamin E group showed better improvement compared to vitamin C and Se.

Antioxidants are used to prevent the increase of oxidative stress, but their simultaneous use has synergistic effects.